Deubiquitinase Archives - Rvpg media

Deubiquitinase USP19 extends the residual enzymatic activity of phenylalanine hydroxylase variants

Cell culture and treatments Human embryonic kidney (HEK293) cells (Korean Cell Bank, Seoul, Korea) were cultured at 37 °C in a humidified atmosphere with 5% CO2 in Dulbecco’s modified Eagle’s medium (GIBCO BRL, Rockville, MD, USA) supplemented with 10% fetal bovine serum (GIBCO BRL) and 1% penicillin and streptomycin (GIBCO BRL). One day before transfection, the … Read more