Deubiquitinase USP19 extends the residual enzymatic activity of phenylalanine hydroxylase variants
Cell culture and treatments Human embryonic kidney (HEK293) cells (Korean Cell Bank, Seoul, Korea) were cultured at 37 °C in a humidified atmosphere with 5% CO2 in Dulbecco’s modified Eagle’s medium (GIBCO BRL, Rockville, MD, USA) supplemented with 10% fetal bovine serum (GIBCO BRL) and 1% penicillin and streptomycin (GIBCO BRL). One day before transfection, the … Read more